|
Method of Use:
For Blot processing, place an intact
blotted membrane bearing antigens, transferred from a gel or applied by
striping, into a Miniblotter®. Ensure that the antigen lines are
perpendicular to the incubation channels machined into the underside of
the top plate. Pipette antibodies into the channels via the access ports
positioned at the ends of the channels. Incubate. Then, wash all the
channels using the plug-in manifold. Introduce secondary antibodies or
other detection reagents into the channels, incubate and wash. Remove
the membrane and transfer to a tray for color development.
To create Bioarrays (Dotblots), apply antigens using the MN45, MN25 .
Wash , remove and rotate the membrane by 90°, place in the appropriate
matching Miniblotter® and add antisera. Incubate, wash and stain as
above..
To perform DNA hybridizations, use the SB10 or SB30 Minislot units to
directly apply target DNA to a blotting membrane using a vacuum source.
Place the membrane in the appropriate Miniblotter® and add DNA probes to
create a grid-like pattern that can generate up to 1,350 hybridization
points. |
